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anti-laminin  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank anti-laminin
    Anti Laminin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laminin/anti-laminin/custom%40c4%4010%2E64898%2F2026%2E07%2E15%2E737746
    Average 94 stars, based on 1 article reviews
    anti-laminin - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: M-cadherin regulates the formation of adherens junctions and secondary myofibers during fetal myogenesis to determine adult myofiber number and muscle mass.
    Article Snippet: Quantification of myofiber size, myofiber type, cell abundance, and cadherin/catenin area was performed using HALO software (Indica Labs) and ImageJ (National Institutes of Health). .. Primary antibodies for immunofluorescence staining were as follows: myosin heavy-chain isoforms (MHC type I, BA-D5; MHC type IIa, SC-71; MHC type IIx, 6H1; MHC type IIb, BF-F3; Developmental Studies Hybridoma Bank [DSHB], University of Iowa), laminin (L9393, Sigma or 1:500 ab11576, Abcam), dystrophin (ab15277, Abcam), myogenin (ab124800, Abcam), M-cadherin (611101, BD Biosciences), Pax7 (Pax7, DSHB), F-actin (1:500; a2228, Thermo Fisher Scientific), pan-cadherin (ab51034, Abcam), non-phosphorylated β-catenin (8814S, Cell Signaling Technology), and Ki67 (ab15580, Abcam). .. The primary antibodies were detected with the appropriate Alexa Fluorophore 488, 546, or 647 secondary antibodies (Thermo Fisher Scientific).

    Staining:

    Article Title: M-cadherin regulates the formation of adherens junctions and secondary myofibers during fetal myogenesis to determine adult myofiber number and muscle mass.
    Article Snippet: Quantification of myofiber size, myofiber type, cell abundance, and cadherin/catenin area was performed using HALO software (Indica Labs) and ImageJ (National Institutes of Health). .. Primary antibodies for immunofluorescence staining were as follows: myosin heavy-chain isoforms (MHC type I, BA-D5; MHC type IIa, SC-71; MHC type IIx, 6H1; MHC type IIb, BF-F3; Developmental Studies Hybridoma Bank [DSHB], University of Iowa), laminin (L9393, Sigma or 1:500 ab11576, Abcam), dystrophin (ab15277, Abcam), myogenin (ab124800, Abcam), M-cadherin (611101, BD Biosciences), Pax7 (Pax7, DSHB), F-actin (1:500; a2228, Thermo Fisher Scientific), pan-cadherin (ab51034, Abcam), non-phosphorylated β-catenin (8814S, Cell Signaling Technology), and Ki67 (ab15580, Abcam). .. The primary antibodies were detected with the appropriate Alexa Fluorophore 488, 546, or 647 secondary antibodies (Thermo Fisher Scientific).

    Immunopeptidomics:

    Article Title: M-cadherin regulates the formation of adherens junctions and secondary myofibers during fetal myogenesis to determine adult myofiber number and muscle mass.
    Article Snippet: Quantification of myofiber size, myofiber type, cell abundance, and cadherin/catenin area was performed using HALO software (Indica Labs) and ImageJ (National Institutes of Health). .. Primary antibodies for immunofluorescence staining were as follows: myosin heavy-chain isoforms (MHC type I, BA-D5; MHC type IIa, SC-71; MHC type IIx, 6H1; MHC type IIb, BF-F3; Developmental Studies Hybridoma Bank [DSHB], University of Iowa), laminin (L9393, Sigma or 1:500 ab11576, Abcam), dystrophin (ab15277, Abcam), myogenin (ab124800, Abcam), M-cadherin (611101, BD Biosciences), Pax7 (Pax7, DSHB), F-actin (1:500; a2228, Thermo Fisher Scientific), pan-cadherin (ab51034, Abcam), non-phosphorylated β-catenin (8814S, Cell Signaling Technology), and Ki67 (ab15580, Abcam). .. The primary antibodies were detected with the appropriate Alexa Fluorophore 488, 546, or 647 secondary antibodies (Thermo Fisher Scientific).

    Article Title: Phospholipid Glutathione Peroxidase Overexpression Mitigates Cancer Cachexia by Protecting Muscle Mass and Lowering Inflammation.
    Article Snippet: .. Sections were incubated in blocking solution (5% Normal Horse Serum PBST) for 30 min at RT and then incubated at 4°C overnight with a cocktail containing antibodies against myosin heavy chains (MHC) and laminin: anti- MHC I (BA- F8), anti- MHC 2A (SC- 71 s) anti- MHC 2B (BF- F3s) (Developmental Studies Hybridoma Bank, Iowa City, IA, USA), and antilaminin (L9393 Sigma). .. Samples were mounted using appropriate medium (ProlLong Glass Antifade Mountant, Invitrogen, P36980) and images were obtained via Nikon Eclipse Ti2 (Nikon, Tokyo Japan) at 10X magnification.

    other:

    Article Title: Contribution of Cardiac CD34⁺ Stromal Cells to Post-Myocardial Infarction Repair in Middle-Aged Rats
    Article Snippet: Laminin, gamma 1 , Mouse , − , IgG2a , 1:100 , PIER , D18 , DSHB, Iowa City, IA.

    Article Title: The skeletal muscle response to high‐intensity training assessed by single‐nucleus RNA‐sequencing is blunted in individuals with type 2 diabetes
    Article Snippet: Laminin (mouse anti‐laminin gamma 1; gene name LAMC1 ; IgG2a; #2E8; DSHB ) , 1:15.

    Next-Generation Sequencing:

    Article Title: Juvenile vs. adult skeletal muscle transplants in the treatment of volumetric muscle loss injury
    Article Snippet: .. Sections were fixed in 4% paraformaldehyde (Thermo Scientific), blocked with 10% normal goat serum (NGS; Thermo Fisher), and incubated overnight with Pax7 (mouse IgG1, 3 μg/mL; DSHB, AB_528428) and laminin (mouse IgG2a, 2 μg/mL; DSHB, AB_2618140). ..

    Article Title: Juvenile vs. adult skeletal muscle transplants in the treatment of volumetric muscle loss injury.
    Article Snippet: .. Sections were fixed in 4% 127 paraformaldehyde (Thermo Scientific), blocked with 10% normal goat serum (NGS; 128 Thermo Fisher), and incubated overnight with Pax7 (mouse IgG1, 3 μg/mL; DSHB, 129 AB_528428) and laminin (mouse IgG2a, 2 μg/mL; DSHB, AB_2618140). ..

    Incubation:

    Article Title: Juvenile vs. adult skeletal muscle transplants in the treatment of volumetric muscle loss injury
    Article Snippet: .. Sections were fixed in 4% paraformaldehyde (Thermo Scientific), blocked with 10% normal goat serum (NGS; Thermo Fisher), and incubated overnight with Pax7 (mouse IgG1, 3 μg/mL; DSHB, AB_528428) and laminin (mouse IgG2a, 2 μg/mL; DSHB, AB_2618140). ..

    Article Title: Nicotinamide and Pyridoxine Supplementation Enhances Muscle Stem Cell Activity and Muscle Regeneration in Humans: A Randomized Placebo-Controlled Clinical Trial of High Force Eccentric Contraction Recovery in Healthy Young Men.
    Article Snippet: .. For Pax7, sections were fixed in 4% PFA (5 mins) before overnight incubation with antibodies against Pax7, laminin and myosin heavy chain I (DSHB, BA-D5, 1:00), visualized by three secondary antibodies (Thermo Fischer Scientific A-21121, A-21144, A-21076). ..

    Article Title: Juvenile vs. adult skeletal muscle transplants in the treatment of volumetric muscle loss injury.
    Article Snippet: .. Sections were fixed in 4% 127 paraformaldehyde (Thermo Scientific), blocked with 10% normal goat serum (NGS; 128 Thermo Fisher), and incubated overnight with Pax7 (mouse IgG1, 3 μg/mL; DSHB, 129 AB_528428) and laminin (mouse IgG2a, 2 μg/mL; DSHB, AB_2618140). ..

    Article Title: Phospholipid Glutathione Peroxidase Overexpression Mitigates Cancer Cachexia by Protecting Muscle Mass and Lowering Inflammation.
    Article Snippet: .. Sections were incubated in blocking solution (5% Normal Horse Serum PBST) for 30 min at RT and then incubated at 4°C overnight with a cocktail containing antibodies against myosin heavy chains (MHC) and laminin: anti- MHC I (BA- F8), anti- MHC 2A (SC- 71 s) anti- MHC 2B (BF- F3s) (Developmental Studies Hybridoma Bank, Iowa City, IA, USA), and antilaminin (L9393 Sigma). .. Samples were mounted using appropriate medium (ProlLong Glass Antifade Mountant, Invitrogen, P36980) and images were obtained via Nikon Eclipse Ti2 (Nikon, Tokyo Japan) at 10X magnification.

    Blocking Assay:

    Article Title: Phospholipid Glutathione Peroxidase Overexpression Mitigates Cancer Cachexia by Protecting Muscle Mass and Lowering Inflammation.
    Article Snippet: .. Sections were incubated in blocking solution (5% Normal Horse Serum PBST) for 30 min at RT and then incubated at 4°C overnight with a cocktail containing antibodies against myosin heavy chains (MHC) and laminin: anti- MHC I (BA- F8), anti- MHC 2A (SC- 71 s) anti- MHC 2B (BF- F3s) (Developmental Studies Hybridoma Bank, Iowa City, IA, USA), and antilaminin (L9393 Sigma). .. Samples were mounted using appropriate medium (ProlLong Glass Antifade Mountant, Invitrogen, P36980) and images were obtained via Nikon Eclipse Ti2 (Nikon, Tokyo Japan) at 10X magnification.



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